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簡要描述：β淀粉樣肽1-42單克隆抗體注意事項(xiàng)；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品型號：GOY-01K0189廠商性質(zhì)：科研更新時(shí)間：2023-10-24 00:00:00訪 問 量：208產(chǎn)品分類
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商品屬性：
貨號 | 產(chǎn)品名稱 | 規(guī)格 |
GOY-01K0189 | β淀粉樣肽1-42單克隆抗體 | 50ul |
GOY-01K0189 | β淀粉樣肽1-42單克隆抗體 | 100ul |
GOY-01K0189 | β淀粉樣肽1-42單克隆抗體 | 200ul |
英文名稱: beta-Amyloid (1-42)
中文名稱: β淀粉樣肽1-42單克隆抗體
別 名；beta Amyloid(1-42); beta Amyloid 1-42;
beta-Amyloid 1-42; Amyloid 1-42; P3(42); A4; AAA; ABETA; ABPP; AD1; Alzheimers
Disease Amyloid Protein; Amyloid B; Amyloid Beta A4 Protein Precursor; Amyloid
Beta; Amyloid of Aging and Alzheimer Disease; APP; APPI; B Amyloid; Beta APP;
Cerebral Vascular Amyloid Peptide; CTFgamma; CVAP; PN II; PN2; PreA4; Protease
nexin II; A beta; A4_HUMAN; Beta-amyloid protein 42.
研究領(lǐng)域；細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) Alzheimer's
抗體來源；Mouse
克隆類型；Monoclonal
克 隆 號2E5
交叉反應(yīng)；(predicted: Human, Mouse, Rat,
)
產(chǎn)品應(yīng)用；WB=1:500-2000
ELISA=1:5000-10000
not yet tested in
other applications.
optimal dilutions/concentrations
should be determined by the end user.
理論分子量；4.4kDa
細(xì)胞定位；細(xì)胞核 細(xì)胞漿 細(xì)胞膜 細(xì)胞外基質(zhì)
性 狀；Liquid
免 疫 原；KLH conjugated synthetic
peptide derived from human beta-Amyloid: 672-713/770 or 1-42/42
亞 型；IgG2b
純化方法；affinity purified by Protein A
緩 沖 液；0.01M PBS(pH7.4)
注意事項(xiàng)；This product as supplied is
intended for research use only, not for use in human, therapeutic or diagnostic
applications.
產(chǎn)品介紹 The cerebral and vascular
plaques associated with Alzheimer's disease are mainly composed of Amyloid beta
peptides. beta Amyloid is derived from cleavage of the Amyloid precursor
protein and varies in length from 39 to 43 amino acids. beta Amyloid [1-40],
beta Amyloid [1-42], and beta Amyloid [1-43] peptides result from cleavage of
Amyloid precursor protein after residues 40, 42, and 43, respectively. The
cleavage takes place by gamma-secretase during the last Amyloid precursor
protein processing step. beta Amyloid [1-40], beta Amyloid [1-42], and beta
Amyloid [1-43] peptides are major constituents of the plaques and tangles that
occur in Alzheimer's disease. beta Amyloid antibodies and peptides have been
developed as tools for elucidating the biology of Alzheimer's disease.
Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Beta-amyloid 42 is a more effective reductant than beta-amyloid 40. Beta-amyloid peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain. The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis. N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6).
實(shí)驗(yàn)流程：
（1）特異性結(jié)合抗原：抗體本身不能直接溶解或殺傷帶有特異抗原的靶細(xì)胞，通常需要補(bǔ)體或吞噬細(xì)胞等共同發(fā)揮效應(yīng)以清除病原微生物或?qū)е虏±頁p傷。然而，抗體可通過與病毒或毒素的特異性結(jié)合，直接發(fā)揮中和病毒的作用。
（2）活補(bǔ)體：IgM、IgG1、IgG2和IgG3可通過經(jīng)典途徑激活補(bǔ)體，凝聚的IgA、IgG4和IgE可通過替代途徑激活補(bǔ)體。
（3）結(jié)合細(xì)胞：不同類別的免疫球蛋白，可結(jié)合不同種的細(xì)胞，參與免疫應(yīng)答。
（4）可通過胎盤及粘膜：免疫球蛋白G（IgG）能通過胎盤進(jìn)入胎兒血流中，使胎兒形成自然被動(dòng)
免疫。免疫球蛋白A（IgA）可通過消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗體分子是一種蛋白質(zhì)，也具有刺機(jī)體產(chǎn)生免疫應(yīng)答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗體對理化因子的抵抗力與一般球蛋白相同：不耐熱，60～70℃即被破壞。各種酶及能使蛋白質(zhì)凝固變性的物質(zhì)，均能破壞抗體的作用?？贵w可被中性鹽類沉淀。在生產(chǎn)上?？捎昧蛩徜@或硫酸鈉從免疫血清中沉淀出含有抗體的球蛋白，再經(jīng)透析法將其純化。
抗體的制備過程：
1.免疫原：普通的大分子蛋白，通過分子克隆構(gòu)建載體并在大腸桿菌中進(jìn)行誘導(dǎo)表達(dá)獲得重組蛋白，純化鑒定后可直接作為免疫原；小分子蛋白或化合物等分子量小，需要偶聯(lián)載體對該分子進(jìn)行改造才能使其成為具有免疫原性的抗原，常見偶聯(lián)載體如BSA、OVA、HAS等。
2. 免疫動(dòng)物：常用于制備抗血清的動(dòng)物有豚鼠、家兔、雞、大小鼠等，大量生產(chǎn)時(shí)需要用到狗、綿羊、山羊等。
3.免疫血清的收集：一般家兔、綿羊、山羊可采用靜動(dòng)脈采血，家兔、豚鼠、大鼠、雞可采用心臟采血，家兔、山羊、綿羊可采用靜脈采血。
4. 免疫血清的純化與鑒定：得到的抗血清需要進(jìn)一步的純化，利用偶聯(lián)了抗原的親和柱進(jìn)行層析，具有高效，特異性強(qiáng)，純度高的特定。接著要鑒定純化蛋白的含量、相對分子的質(zhì)量、純度以及特異性。
5.β淀粉樣肽1-42單克隆抗體免疫血清的保存：抗體一般比較穩(wěn)定，在-80℃ ～-20 ℃可以保存約5年而不會(huì)影響效價(jià)，而真空干燥保存時(shí)間可以更久。保存前需經(jīng)除菌并添加防腐劑。
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