簡要描述:魚雌激素請每次測定的同時做標(biāo)準(zhǔn)曲線,做復(fù)孔。如標(biāo)本中待測物質(zhì)含量過高(樣本OD值大于標(biāo)準(zhǔn)品孔*孔的OD值),請先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測定,計(jì)算時請Z后乘以總稀釋倍數(shù)(×n×5)。
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魚雌激素尿液:請收集清晨*次尿液(中段尿),或24小時尿液,2000 x g離心5分鐘后收集上清,并將標(biāo)本保存于-20℃,且應(yīng)避免反復(fù)凍融。 其它生物標(biāo)本:請000 x g離心20分鐘,取上清即可檢測,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融。www.bjjzst.com
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